Metabolic Characteristics of Isolated Rabbit Heart Mitochondria
نویسندگان
چکیده
منابع مشابه
Regulation of pyruvate oxidation in isolated rabbit heart mitochondria.
Evidence is presented in support of the hypothesis that the pyruvate dehydrogenase multienzyme complex of intact rabbit heart mitochondria may be regulated by a phosphorylation-dephosphorylation mechanism. Mitochondria incubated in the presence of pyruvate plus L-malate and either ADP or uncoupler exhibit nearly identical, rapid rates of pyruvate oxidation but possess markedly different ATP lev...
متن کاملMalic Enzymes of Rabbit Heart Mitochondria
Two distinct malic enzymes have been separated from rabbit heart mitochondria. Both enzymes require Mn2+ or Mg2+ for activity. One of these enzymes (Enzyme 1) catalyzes the oxidative decarboxylation of malate in the presence of either NAD+ or NADP+, the former being much the more effective. After purification lOOto ZOO-fold by Sephadex and DEAE-cellulose chromatography and removal of contaminat...
متن کاملMETABOLIC STUDlES ON HEART MITOCHONDRIA*
Relatively few investigations have been made on the oxidation of pyruvate by heart mitochondria. Guinea pig heart mitochondria (1) and rabbit heart cyclophorase preparations (2, 3) have been found to oxidize pyruvate, but in no case has a detailed investigation been made of the mechanisms involved and the requirements for the operation of the complete tricarboxylic acid cycle. On the other hand...
متن کاملMetabolism in Isolated Rabbit Heart
To investigate whether energy derived from glycolytic and oxidative metabolism are preferentially used for different functions in heart, tension, intracellular potential, and extracellular [K+] and pH (using triple barrel K/pH electrodes, tip diameter 0.5 mm) were monitored in isolated arterially perfused rabbit interventricular septa during exposure to hypoxia and metabolic inhibitors. Myocard...
متن کاملMorphology of Isolated Rabbit Heart Muscle Mitochondria and the Oxidation of Extramitochondrial Reduced Diphosphopyridine Nucleotide
The morphology of rabbit heart muscle mitochondria isolated in several media has been compared by electron microscopy. The internal structure of isolated mitochondria differs from that of in situ mitochondria, with the type and degree of alteration depending on the isolation medium. Examination of the isolated mitochondria after incubation revealed that additional morphological changes occurred...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1965
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)97583-6